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Batch fermentation. Nutrients and bacterial strains are added for cultivation at once until they are released, and there is no material exchange with the outside except for air entering and exhaust gas being discharged. Features: disposable; During the fermentation process, nutrients continuously decrease, microorganisms continue to proliferate, and the environment is unstable; The four stages of microbial growth are evident. Application: Widely used.
2020
06-23
Working principle of airlift fermentor
An air lift loop reactor is a type of reactor that does not have a stirrer, but has a central guide tube that divides the fermentation broth into an ascending zone (inside the guide tube) and a descending zone (outside the guide tube). An air nozzle or a circular air distribution tube is installed at the lower part of the ascending zone, and there are many spray holes below the air distribution tube.
2020
06-23
Mechanical stirring and ventilation fermentation tanks are widely used in biotechnology factories. According to incomplete statistics, they account for 70% to 80% of the total number of fermentation tanks, so they are often referred to as universal fermentation tanks. These types of fermentation tanks are mostly used for ventilated fermentation, relying on compressed air and stirring impellers to achieve mixing and dissolved oxygen mass transfer of the fermentation broth, while enhancing heat transfer.
2020
06-23
Formation of fermentation foam and selection of defoamer
To break the foam, the defoamer should be concentrated on the foam film. For defoamers, they should be concentrated and concentrated in an instant, while for foam suppressants, they should be kept in this state regularly. So defoamers are in a supersaturated state in foaming liquids, and only insoluble or poorly soluble ones are prone to reaching supersaturation.
2020
06-23
SOP for fermentation tank cleaning
To clean tanks below 50L, loosen the screws and connections on the top cover, remove the fermentation tank top cover, remove the inoculation cap, feeding hole plug, defoamer probe, and reserved hole plug, clean and wipe each component with a cloth, and rinse with tap water. Use a soft bristled brush to clean the lid, the interface between the lid and the tank, the inner wall of the tank, and the seal of the mixer. Rinse off any residue and liquid with tap water, and collect the rinsing water in a bucket during rinsing.
2020
06-23
Notes on maintenance and operation of fermentation tank
The precision filter for fermentation tanks generally has a lifespan of six months. If the filtration resistance is too high or the filtration capacity is lost, which affects normal production, it needs to be cleaned or replaced (it is recommended to replace it directly without cleaning, as the performance of the filter cannot be reliably guaranteed after cleaning operation).
2020
06-23
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